Possibilities and limitations of vegetative propagation in breeding and mass propagation of Norway spruce

The use of vegetative mass propagation in practical forestry with Norway spruce (Picea abies (L.) Karst.) is limited at present, although its potential to deliver high genetic gains is obvious. The objective of this thesis was to study possibilities and limitations of vegetative propagation when applied in different parts of a breeding/mass propagation system for Norway spruce. Two vegetative propagation methods were studied: somatic embryogenesis and cutting propagation. Somatic embryogenesis was accompanied by losses of genotypes during the propagation process. The embryogenic response at proliferation and maturation was under family control, while germination was obtained for all families. Parental effects on proliferation and maturation were found for male parents but not for female. However, no correlations between embryogenic characters and breeding goal traits could be detected on parental level. Shortening of treatment with abscisic acid (ABA) during somatic embryo development gave pronounced positive effects on height growth of regenerated plants. An improved protocol, including five weeks ABA treatment and root development in liquid medium significantly improved performance of the resulting plants. The number of plants with lateral roots at the time of ex vitro transfer increased substantially with this protocol. Lateral roots at ex vitro transfer were shown to be a marker for good height growth and clonal uniformity during the next two years. Selection for height of cutting propagated clones in the nursery resulted in low responses in height after six years in field. The likely reason for this was low correlations between nursery traits and field traits. Genotype x environment interactions in the studied clonal test series varied from close to zero to more than 50% of the clone component. A tendency towards increased interaction components with age was obtained in one of the series. In situations with large genotype x environment interactions, clonal stability over sites should be included in the selection criteria

Analysis of OFF research topics in CORE Organic participating countries

This analysis of OFF research in the participating countries of CORE Organic is based on titles of projects running during the time period 2000-2007, with some variation from country to country. Lists of project titles were taken from country reports

Programmed cell death and genetic stability in conifer embryogenesis

Somatic embryogenesis, the generation of embryos from somatic cells, is a valuable tool for studying embryology. In addition, somatic embryos can be used for large-scale vegetative propagation, an application of great interest for forestry. A critical event during early embryo differentiation in conifers is the apical basal polarization, which proceeds through the establishment of two embryonic parts: the proliferating embryonal mass and the terminally differentiated suspensor. The development of both parts is strictly coordinated and imbalance causes embryonic defects. The suspensor cells are eliminated by programmed cell death (PCD). In animals, caspase family proteases are the main executioners of PCD. In this work we have used synthetic peptide substrates containing caspase recognition sites and corresponding specific inhibitors to analyse the role of caspase-like activity during early embryo differentiation in Norway spruce (Picea abies L. Karst.). We found that VEIDase is the principal caspase-like activity. This activity is localized specifically in suspensor cells, and its inhibition prevents normal embryo development by blocking the suspensor differentiation. The in vitro VEIDase activity was shown to be highly sensitive to pH, ionic strength, temperature and zinc concentration. In vivo studies with Zinquin, a zinc-specific fluorescent probe, revealed a high accumulation of intracellular free zinc in the embryonal masses and an abrupt decrease in the suspensor. Increased zinc concentration in the culture medium suppresses terminal differentiation and PCD of the suspensor. In accordance, exposure of early embryos to TPEN, a zinc-specific chelator, induces ectopic cell death affecting embryonal masses. This establishes zinc as an important factor affecting cell fate specification during plant embryogenesis. Before somatic embryos can be accepted for clonal propagation it is important to show that the regenerated plants have similar growth to that of seedlings and are genetically uniform. The genetic integrity during zygotic and somatic embryogenesis in Norway spruce and Scots pine (Pinus sylvestris L.) was investigated by comparing the stability of variable nuclear microsatellite loci. The stability varied significantly among families in both species during somatic embryogenesis. Scots pine families showing low genetic stability during establishment of embryogenic cultures had a higher embryogenic potential than those that were genetically more stable. In contrast, embryo development was suppressed in genetically unstable families. The stability of microsatellites was in general higher in zygotic embryos than in somatic embryos. No deviation in growth was observed in somatic embryo plants of Norway spruce carrying mutated microsatellites

Animal breeding in organic farming

After a general introduction into the available breeding techniques for animal breeding and an overview of the organic principles, points for discussion are identified and scenario's for organically accepted breeding methods are discussed

Towards F1 Hybrid Seed Potato Breeding

Compared to other major food crops, progress in potato yield as the result of breeding efforts is very slow. Genetic gains cannot be fixed in potato due to obligatory out-breeding. Overcoming inbreeding depression using diploid self-compatible clones should enable to replace the current method of out-breeding and clonal propagation into an F1 hybrid system with true seeds. This idea is not new, but has long been considered unrealistic. Severe inbreeding depression and self-incompatibility in diploid germplasm have hitherto blocked the development of inbred lines. Back-crossing with a homozygous progenitor with the Sli gene which inhibits gametophytic self-incompatibility gave self-compatible offspring from elite material from our diploid breeding programme. We demonstrate that homozygous fixation of donor alleles is possible, with simultaneous improvement of tuber shape and tuber size grading of the recipient inbred line. These results provide proof of principle for F1 hybrid potato breeding. The technical and economic perspectives are unprecedented as these will enable the development of new products with combinations of useful traits for all stakeholders in the potato chain. In addition, the hybrid’s seeds are produced by crossings, rendering the production and voluminous transport of potato seed tubers redundant as it can be replaced by direct sowing or the use of healthy mini-tubers, raised in greenhouses

Neglected and Underutilized Plant Species in Horticultural and Ornamental Systems

This Special Issue contributes to filling knowledge gaps regarding NUS in horticultural and ornamental systems, as well as in landscapes, by collecting original research papers dealing with the relevance of NUS to the following topics: biodiversity and conservation; genetics and breeding; characterization, propagation, and ecophysiology; cultivation techniques and systems; landscape protection and restoration; product and process innovations; biochemistry and composition; and postharvest factors affecting their end-use quality

Terminology server for improved resource discovery: analysis of model and functions

This paper considers the potential to improve distributed information retrieval via a terminologies server. The restriction upon effective resource discovery caused by the use of disparate terminologies across services and collections is outlined, before considering a DDC spine based approach involving inter-scheme mapping as a possible solution. The developing HILT model is discussed alongside other existing models and alternative approaches to solving the terminologies problem. Results from the current HILT pilot are presented to illustrate functionality and suggestions are made for further research and development

Animal breeding in organic farming:Discussion paper

It is uncertain whether animals which have been bred for conventional production are capable of optimum performance in organic conditions. In conventional agriculture there is a movement towards maximum control of production conditions in order to optimise animals' yield in intensive production systems. By contrast, organic agriculture is based on natural processes and closed cycles, and takes into account the underlying connections between production factors. Following organic ideology, production capacity should be curtailed by acting in accordance with guiding principles such as naturalness, animal welfare, efficient use of fossil fuels in the farm cycle, and agri-biodiversity (IFOAM, 1994). Organic production should be tied to the land, with farms preferably being self-sufficient mixed farms with closed cycles. An additional point of concern are the reproduction techniques used in conventional breeding. Artificial insemination (AI) and embryo transfer (ET) are commonplace in conventional animal breeding. But these techniques are 'artificial' and they deprive animals of natural mating behaviour and negatively affect the animals' welfare and integrity. By bringing in animals from conventional agriculture, organic farmers are indirectly making use of these techniques. These and other concerns have led to the project 'Organic breeding: a long way to go', which aims to lay down clear visions and an action plan for an organic breeding system